JPAC Joint United Kingdom (UK) Blood Transfusion and Tissue Transplantation Services Professional Advisory Committee

16.5: Testing of HLA genes and gene products

DNA-based methods must identify all HLA alleles included in the most recent WHO Nomenclature Committee for Factors of the HLA System Report2 and Nomenclature for Factors of the HLA System, 2010.3 Alleles should be reported either as individual alleles or as allele groups with two digits (first field). Definitions of allelic, high, intermediate and low resolution molecular HLA typing are available through EFI.

The minimum level of resolution by serological typing is given in Table 16.1. Typing to the level of broad specificities is acceptable but the higher level to include the split specificities, as indicated, is recommended. HLA-C types Cw12 and Cw14 to Cw18 have not been formally designated as recognised antigens and may not be identified serologically.

 

Table 16.1 HLA antigens that are defined by serological typing (with broad specificities shown in brackets)

HLA-A

HLA-B

HLA-C

HLA-DR

HLA-DQ

A1

B7

Cw1

DR1

DQ5 (1)

A2

B8

Cw2

DR15 (2)

DQ6 (1)

A3

B13

Cw9 (3)

DR16 (2)

DQ2

A11

B18

Cw10 (3)

DR17 (3)

DQ7 (3)

A23 (A9)

B27

Cw4

DR18 (3)

DQ8 (3)

A24 (A9)

B35

Cw5

DR4

DQ9 (3)

A25 (A10)

B37

Cw6

DR11 (5)

DQ4

A26 (A10)

B38 (B16)

Cw7

DR12 (5)

 

A29 (A19)

B39 (B16)

Cw8

DR13 (6)

 

A30 (A19)

B41

Cw12#

DR14 (6)

 

A31 (A19)

B42

Cw14#

DR7

 

A32 (A19)

B45

Cw15#

DR8

 

A33 (A19)

B44

Cw16#

DR9

 

A34 (A10)

B46

Cw17#

DR10

 

A36

B47

Cw18#

DR103

 

A43

B48

 

 

 

A66 (A10)

B49 (B21)

 

DR51

 

A68 (A28)

B50 (B21)

 

DR52

 

A69 (A28)

B51 (B5)

 

DR53

 

A74 (A19)

B52 (B5)

 

 

 

A80

B53

 

 

 

 

B54 (B22)

 

 

 

 

B55 (B22)

 

 

 

 

B56 (B22)

 

 

 

 

B57 (B17)

 

 

 

 

B58 (B17)

 

 

 

 

B59

 

 

 

 

B60 (B40)

 

 

 

 

B61 (B40)

 

 

 

 

B62 (B15)

 

 

 

 

B63 (B15)

 

 

 

 

B64 (B14)

 

 

 

 

B65 (B14)

 

 

 

 

B67

 

 

 

 

B71 (B70)

 

 

 

 

B72 (B70)

 

 

 

 

B73

 

 

 

 

B75 (B15)

 

 

 

 

B76 (B15)

 

 

 

 

B77 (B15)

 

 

 

 

B78

 

 

 

 

B81

 

 

 

 

Bw4

 

 

 

 

Bw6

 

 

 

# The products of the Cw12 and Cw14 to Cw18 genes have not been formally designated as recognised antigens and might not be identified serologically.